Tuberculosis is a bacterial infection caused by Mycobacterium tuberculosis and primarily affects the lungs. TB is a major global health problem, causing significant morbidity and mortality worldwide. TB is a leading cause of death worldwide, particularly in low- and middle-income countries. We conduct genotypic analyses and, in collaboration with our international partners, have carried out studies in Cambodia aimed at optimizing TB case detection, treatment, and prevention strategies to bridge gaps in the healthcare system.